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Exam 10: DNA Structure and Analysis

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Question 21

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The base content of a sample of nucleic acid is as follows: A = 31%,G = 31%,T = 19%,C = 19%.What conclusion should be drawn from this information?

A) This nucleic acid is double- stranded DNA.
B) This nucleic acid is made of RNA.
C) The purine/pyrimidine ratio in this molecule fits Chargaff's data.
D) The strands in this molecule are parallel rather than antiparallel.
E) This nucleic acid is single- stranded DNA.

F) A) and D)
G) A) and E)

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Question 22

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The covalent linkage between the monomers in a dinucleotide is an______.

A) phosphodiester bond
B) peptide bond
C) ester bond
D) ionic bond
E) disulfide bond

F) D) and E)
G) All of the above

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Question 23

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In the mid- 1940s,genetic information was known to consist of__________ .

The figure below shows a representative dinucleotide: -Spleen diesterase is an enzyme that breaks the covalent bond that connects the phosphate to the 5' carbon.If the dinucleotide is digested with spleen diesterase,to which base and to which carbon on the sugar is the phosphate attached?

Arrange the principal forms of RNA in order of abundance from LEAST abundant to MOST abundant.

Compared with B- DNA,A- DNA is ______compact,has ______ base pairs per turn,and is a ______ helix.

Who discovered that DNA was the genetic material or "transforming principle" that could convert nonvirulent R- type Diplococcus pneumoniae bacterium to the virulent S- type?

How do urea and NaCl influence temperature- induced DNA melting?

Regarding the structure of DNA,the covalently arranged combination of a deoxyribose and a nitrogenous base would be called an______ .

In base pair between a guanine and a cytosine, ______hydrogen bonds form between a partially positive hydrogen on the guanine and a more electronegative atom on the cytosine.

What is the difference between a polynucleotide and an oligonucleotide?

The classic Hershey and Chase 1952) experiment that offered evidence in support of DNA being the genetic material in bacteriophages made use of which of the following labeled components ?

A particular__________ carry the information for making a particular protein,but__________ can be used to make any protein.

Consider the following two statements about DNA melting temperature: 1) The melting temperature of a sample of DNA is the temperature at which 50% of the double helices in the sa have completely denatured. 2) A molecule with 53% G—C base pairs would have a higher melting temperature than a molecule of equivale length with 53% A—T base pairs. Determine which one of the statements below is CORRECT.

Any two nucleic acid fragments will spontaneously hybridize if they encounter each other as long as they are ______and ______.

Why would D- DNA and E- DNA be unlikely to be found in the genomes of living organisms?

What does it mean for a double helix of DNA to be antiparallel and complementary?

The figure below shows a representative dinucleotide: - This figure represents __________,and the arrow is closest to the__________ end.

Explain the tetranucleotide hypothesis and its relationship to the substance referred to as nuclein.

Genetic functions in a eukaryote can be observed in __________.