Question 1

Which cell can transfer chromosomal and plasmid genes to a recipient cell by conjugation?

Accepted Answer

A)  F⁺ cell
B)  F⁻ cell
C)  Hfr cell
D)  R cell
E)  B cellF) A) and E)
G) All of the above

Question 2

If you start with three double-stranded DNA fragments,after four cycles of PCR you will have ________ fragments.

Accepted Answer

A)  12
B)  24
C)  27
D)  48
E)  81F) B) and C)
G) None of the above

Question 3

Which of the following statements best describes the initiation of translation?

Accepted Answer

A)  The mRNA containing the start codon,AUG,sits at the P site and forms a complex with the corresponding tRNA,and the large and small ribosomal subunits.
B)  A tRNA with the anticodon,AUG,enters the ribosomal complex and binds to the mRNA at the A site.
C)  The large and small ribosomal subunits scan the mRNA in the 3'-5' direction until the promoter is reached.
D)  The mRNA attaches to the large ribosomal subunit and once the start codon reaches the A site,the tRNA binds and the small subunit completes the complex.E) C) and D)
F) B) and D)

Question 4

Which statement best describes the flow of genetic information in a cell?

Accepted Answer

A)  DNA codes for RNA,which codes for proteins and some information from RNA flows back to DNA in a regulatory role.
B)  DNA codes for RNA,which codes for proteins.
C)  DNA synthesizes proteins.
D)  RNA directs the synthesis of DNA,which codes for proteins.E) C) and D)
F) B) and C)

Question 5

DNA polymerase III ________.

Accepted Answer

A)  is needed for adding nucleotides during mRNA synthesis
B)  synthesizes new DNA only in the 5' to 3' direction
C)  cannot add nucleotides to the lagging strand
D)  synthesizes an RNA primer
E)  All of the choices are correct.F) A) and B)
G) A) and C)

Question 6

Synthesis of an inducible enzyme requires ________.

Accepted Answer

A)  repressor alone bound to operator
B)  substrate bound to repressor
C)  substrate bound to promoter
D)  corepressor and repressor binding to operator
E)  None of the choices are correct.F) A) and E)
G) A) and D)

Question 7

The site where the old DNA strands separate and new DNA strands will be synthesized is called the ________.

Accepted Answer

A)  primer
B)  Okazaki fragment
C)  template
D)  rolling circle
E)  replication forkF) A) and B)
G) B) and D)

Question 8

A three base sequence on mRNA is called a(n) ________.

Accepted Answer

A)  codon
B)  exon.
C)  anticodon
D)  intronE) A) and B)
F) C) and D)

Question 9

An RN working in a research unit is responsible for data and specimen collection for multiple research studies. A patient is admitted as a participant in a study focused on the role of tRNA mutations in Alzheimer's disease. The 78-year-old male is accompanied by his wife and daughter. Prior to obtaining the first blood specimen, the study coordinator provides education regarding the importance of the study and the overall process of protein synthesis.
-During protein synthesis,the final mRNA codon on the strand,UAA,recruits which of the following tRNA molecules to the ribosomal complex?

Accepted Answer

A)  tRNA carrying methionine
B)  tRNA carrying proline
C)  tRNA carrying arginine
D)  No corresponding tRNA exists for this codon.E) C) and D)
F) A) and D)

Question 10

EcoRI and HindIII are ________.

Accepted Answer

A)  palindromes
B)  reverse transcriptase
C)  restriction endonucleases
D)  ligases
E)  DNA polymerasesF) None of the above
G) A) and C)

Question 11

Bacterial conjugation involves ________.

Accepted Answer

A)  bacteriophage carrying donor DNA to the recipient cell
B)  a donor cell with a plasmid that synthesizes a pilus
C)  naked DNA fragments from a lysed donor cell taken up by a recipient cell
D)  new progeny cells with genes from two parent bacterial cells
E)  None of the choices are correct.F) A) and E)
G) All of the above

Question 12

Transcription occurs in the nucleus or at the nucleoid.

Accepted Answer

A) True 
 B)False

Question 13

The duplication of a cell's DNA is called ________.

Accepted Answer

A)  mitosis
B)  replication
C)  transcription
D)  translation
E)  mutationF) A) and D)
G) B) and D)

Question 14

The development of virulent,toxin-producing bacterial strains due to the presence of a temperate phage can occur in ________.

Accepted Answer

A)  bacterial conjugation
B)  transformation
C)  generalized transduction
D)  specialized transduction
E)  All of the choices are correct.F) A) and E)
G) C) and D)

Question 15

What type of mutation alters the base,but not the amino acid being coded for?

Accepted Answer

A)  Silent
B)  Back
C)  Point
D)  Nonsense
E)  MissenseF) None of the above
G) All of the above

Question 16

DNA polymerases used in PCR ________.

Accepted Answer

A)  use an RNA template to make complementary DNA
B)  must remain active at very cold temperatures
C)  include Taq polymerase
D)  are labeled with fluorescent dyes
E)  All of the choices are correctF) A) and D)
G) B) and D)

Question 17

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Accepted Answer

A)  Missense
B)  Nonsense
C)  Insertion
D)  Deletion
E)  SilentF) A) and C)
G) C) and E)

Question 18

DNA Polymerase I ________.

Accepted Answer

A)  adds bases to new DNA chain
B)  supercoils DNA
C)  removes primers
D)  unzips DNA
E)  synthesizes RNA primerF) C) and D)
G) None of the above

Question 19

The enzymes that can proofread replicating DNA,detect incorrect bases,excise them,and correctly replace them are ________.

Accepted Answer

A)  DNA ligases
B)  DNA polymerases
C)  DNA helicases
D)  DNA gyrases
E)  primasesF) B) and C)
G) B) and D)

Question 20

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)  Add DNA polymerase and nucleotides at 72°C
B)  Cool DNA to between 50°C and 65°C
C)  Add primers
D)  Heat target DNA to 94°C
E)  Repeat the cycle of heating and coolingF) A) and B)
G) A) and C)

Exam 8: Microbial Genetics and Genetic Engineering

Bacterial conjugation involves ________.

Correct Answer
verified

Transcription occurs in the nucleus or at the nucleoid.

Correct Answer
verified

The development of virulent,toxin-producing bacterial strains due to the presence of a temperate phage can occur in ________.

Correct Answer
verified

Synthesis of an inducible enzyme requires ________.

Correct Answer
verified

Which cell can transfer chromosomal and plasmid genes to a recipient cell by conjugation?

Correct Answer
verified

Correct Answer
verified

A three base sequence on mRNA is called a(n) ________.

Correct Answer
verified

Which PCR step causes the denaturation of double-stranded DNA?

Correct Answer
verified

EcoRI and HindIII are ________.

Correct Answer
verified

The duplication of a cell's DNA is called ________.

Correct Answer
verified

What type of mutation alters the base,but not the amino acid being coded for?

Correct Answer
verified

DNA polymerases used in PCR ________.

Correct Answer
verified

If you start with three double-stranded DNA fragments,after four cycles of PCR you will have ________ fragments.

Correct Answer
verified

DNA polymerase III ________.

Correct Answer
verified

The enzymes that can proofread replicating DNA,detect incorrect bases,excise them,and correctly replace them are ________.

Correct Answer
verified

DNA Polymerase I ________.

Correct Answer
verified

Which statement best describes the flow of genetic information in a cell?

Correct Answer
verified

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Correct Answer
verified

The site where the old DNA strands separate and new DNA strands will be synthesized is called the ________.

Correct Answer
verified

Which of the following statements best describes the initiation of translation?

Correct Answer
verified

Exam 8: Microbial Genetics and Genetic Engineering

Bacterial conjugation involves ________.

Correct AnswerverifiedShow Answer

Transcription occurs in the nucleus or at the nucleoid.

Correct AnswerverifiedShow Answer

The development of virulent,toxin-producing bacterial strains due to the presence of a temperate phage can occur in ________.

Correct AnswerverifiedShow Answer

Synthesis of an inducible enzyme requires ________.

Correct AnswerverifiedShow Answer

Which cell can transfer chromosomal and plasmid genes to a recipient cell by conjugation?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

A three base sequence on mRNA is called a(n) ________.

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

EcoRI and HindIII are ________.

Correct AnswerverifiedShow Answer

The duplication of a cell's DNA is called ________.

Correct AnswerverifiedShow Answer

What type of mutation alters the base,but not the amino acid being coded for?

Correct AnswerverifiedShow Answer

DNA polymerases used in PCR ________.

Correct AnswerverifiedShow Answer

If you start with three double-stranded DNA fragments,after four cycles of PCR you will have ________ fragments.

Correct AnswerverifiedShow Answer

DNA polymerase III ________.

Correct AnswerverifiedShow Answer

The enzymes that can proofread replicating DNA,detect incorrect bases,excise them,and correctly replace them are ________.

Correct AnswerverifiedShow Answer

DNA Polymerase I ________.

Correct AnswerverifiedShow Answer

Which statement best describes the flow of genetic information in a cell?

Correct AnswerverifiedShow Answer

If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T?

Correct AnswerverifiedShow Answer

The site where the old DNA strands separate and new DNA strands will be synthesized is called the ________.

Correct AnswerverifiedShow Answer

Which of the following statements best describes the initiation of translation?

Correct AnswerverifiedShow Answer

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