Question 1

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Accepted Answer

A)  PCR
B)  genetic engineering
C)  antisense technology
D)  microarray analysis
E)  DNA sequencingF) C) and D)
G) A) and D)

Question 2

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 3

Transgenic animals are _____ modified organisms.

Accepted Answer

genetically

Question 4

The fluorescent in situ hybridization (FISH)  probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) C) and D)
G) D) and E)

Question 5

The polymerase chain reaction (PCR)  has been used in all of the following fields, except

Accepted Answer

A)  forensics.
B)  evolutionary studies.
C)  gene mapping.
D)  medicine.
E)  None of the choices; it is useful for all these fields.F) A) and D)
G) D) and E)

Question 6

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 7

All of the following are correct about DNA fingerprinting, except

Accepted Answer

A)  it displays the unique pattern of an individual's DNA fragments.
B)  it can be used to analyze evidence when only minute amounts of DNA are found.
C)  it cannot be used on specimens older than five years.
D)  it is routine to use it as trial evidence in the U.S.
E)  it can be used on hair and saliva.F) A) and E)
G) B) and E)

Question 8

The enzyme___ adds three nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated

Accepted Answer

A)  DNA polymerase III, 3' →\rarr→ 5'
B)  DNA polymerase I, 3' →\rarr→ 5'
C)  DNA polymerase III, 5' →\rarr→ 3'
D)  DNA polymerase I, 5' →\rarr→ 3'
E)  None of the above F) A) and B)
G) A) and D)

Question 9

The function of the dideoxy (dd)  nucleotides that are used in the Sanger method of DNA sequencing is to

Accepted Answer

A)  denature DNA into single strands.
B)  serve as primers.
C)  be a fluorescent tag.
D)  incorporate into newly replicated DNA strands and stop elongation.
E)  None of the choices are correct.F) None of the above
G) A) and E)

Question 10

When DNA is heated to just below boiling (90 ∘\circ∘ C - 95 ∘\circ∘ C) ,

Accepted Answer

A)  the sugar phosphate backbone of the DNA strands breaks.
B)  the sugar, phosphate and base come apart in each nucleotide.
C)  the helix unwinds but hydrogen bonds between the bases remain intact.
D)  the two DNA strands separate completely.
E)  Nothing happens until the boiling point is reached (100 ∘\circ∘ 
C)  . F) None of the above
G) A) and B)

Question 11

If you have the sequence of mRNA that is AUAUGC, then the DNA antisense strand is ____.

Accepted Answer

A)  AUAUGC
B)  UAUACG
C)  TATACG
D)  CGUAUAE) A) and B)
F) A) and C) C

Question 12

EcoRI and HindIII are

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) A) and D)
G) A) and B)

Question 13

Two different nucleic acids can _____ by uniting at their complementary sites.

Accepted Answer

The answer of Two different nucleic acids can _____ by...

Question 14

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 15

Gene probes can be labeled for detection with

Accepted Answer

A)  enzymes.
B)  fluorescent dyes.
C)  radioisotopes.
D)  All of the choices are correct.
E)  None of the choices are correct.F) C) and D)
G) B) and C) D

Question 16

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Accepted Answer

A)  human growth hormone
B)  Factor VIII
C)  insulin
D)  tissue plasminogen activator
E)  All of the choices are correct.F) D) and E)
G) A) and E)

Question 17

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

Accepted Answer

1. Start by growing E. coli cells in a l...

Question 18

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 19

DNA strands can be clipped crosswise at selected positions by using enzymes called

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) All of the above
G) B) and D)

Question 20

Sequencing a gene automatically identifies which gene it is.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Correct AnswerverifiedShow Answer

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

Transgenic animals are _____ modified organisms.

Correct Answerverifiedgenetically

The fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Correct AnswerverifiedShow Answer

The polymerase chain reaction (PCR) has been used in all of the following fields, except

Correct AnswerverifiedShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

All of the following are correct about DNA fingerprinting, except

Correct AnswerverifiedShow Answer

The enzyme___ adds three nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated

Correct AnswerverifiedShow Answer

The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to

Correct AnswerverifiedShow Answer

When DNA is heated to just below boiling (90 ∘\circ∘ C - 95 ∘\circ∘ C) ,

Correct AnswerverifiedShow Answer

If you have the sequence of mRNA that is AUAUGC, then the DNA antisense strand is ____.

Correct AnswerverifiedC

EcoRI and HindIII are

Correct AnswerverifiedShow Answer

Two different nucleic acids can _____ by uniting at their complementary sites.

Correct AnswerverifiedShow Answer

When DNA is heated, the two strands will separate.

Correct AnswerverifiedShow Answer

Gene probes can be labeled for detection with

Correct AnswerverifiedD

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Correct AnswerverifiedShow Answer

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

Correct AnswerAnswered by ExamLex AI1. Start by growing E. coli cells in a l...View AnswerShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called

Correct AnswerverifiedShow Answer

Sequencing a gene automatically identifies which gene it is.

Correct AnswerverifiedShow Answer

Correct Answer
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genetically

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The enzyme_ adds three nucleotides of DNA in the _direction when DNA is being semi-conservatively replicated

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When DNA is heated to just below boiling (90^$\circ$C - 95^$\circ$C) ,

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C

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D

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Answered by ExamLex AI
1. Start by growing E. coli cells in a l...
View Answer

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